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. 2022 Jan 7;12:143. doi: 10.1038/s41598-021-03744-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Injured nerve fibers in superficial cerebellar white matter. (a) Silver staining identifies injured nerve fibers (black, red arrowheads) and cell nuclei (brown) in sections taken ipsilateral and contralateral to blast impact. Heads were either fixed in place or allowed to move laterally during blast exposure, and brains were harvested at the indicated time points. Sections from control (sham blast exposure) rats showed no detectable silver staining. Scale bar = 30 µm. Images are representative of n = 3 rats treated under each condition and time point. (b) Quantification of silver-stained neurites (means ± s.e.m per field; n = 3). In both the head-fixed and head-movement conditions, the number of injured neurites was increased relative to the sham condition. (p < 0.01 by the Mann–Whitney test). Differences between the two sides and between the four time points assessed were not statistically different in either condition.