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. 2022 Jan 7;12:74. doi: 10.1038/s41598-021-03832-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Activated HIF-1α with its increased protein expression in the heart primordium after heartbeat initiation. (A) DNA-binding activity of HIF-1α in the nuclear fraction in the pre- and post-heartbeat groups (N = 6). (B,C) Representative images of Western blots (B) and densitometry (C) of HIF-1α, HIF-1β, PHD1, and pVHL in the pre- and post-heartbeat group (N = 5–6). (D,E) Representative images of Western blots (D) and densitometry (E) of PHD2 in the pre- and post-heartbeat groups (N = 6). (F) Relative gene expression levels of HIF-1α, VHL, and PHD2 that were obtained by microarray analysis in the pre- and post-heartbeat groups. *p < 0.05 with Welch's t-test. N.S.: not significant. Full-length blots/gels are presented in Supplementary Figure S1.