Figure 3.

RocA increases the expression of CXCL10 in NSCLC cells independent of autophagy inhibition. A, LLC cells were subcutaneously inoculated onto the upper back of C57BL6 mice on day 0, and 1 mg/kg of RocA was administered by i.p. injection every 2 days from day 3. Mice were sacrificed on day 14, and tumors were isolated and used for RNA-Seq. A, DEGs related to NK activation and traffic were richened. B-D, The expressions of CCL5, CXCL10, and IFN-γ in tumors were detected by real-time PCR. E, The expression of CD107α on the surface of NK cells was analyzed by flow-cytometry. A549 (F), H1299 (G), H1975 (H), and LLC (I) cells were exposed to different concentrations (0, 12.5, and 25 nM) of RocA for 24 h, and then the expression of CXCL10 was analyzed by real-time PCR. J, ULK1^WT, and ULK1^KO H1299 cells were exposed to different concentrations (0, 12.5, and 25 nM) of RocA for 24 h, and then the expression of CXCL10 was analyzed by real-time PCR. K, A549, H1299, and H1975 cells were treated with or without 25 nM RocA in the presence or absence of 10µM SP600125 for 24 h, and then the expression of CXCL10 was analyzed by real-time PCR. Data were pooled from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, non-statistical significance.