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. 2022 Jan 1;18(2):585–598. doi: 10.7150/ijbs.65019

Figure 5.

Figure 5

RocA activates the cGAS-STING signaling pathway and increases the expressions of CCL5 and CXCL10 depending on such pathway. A, A549, H1299, and H1975 cells were exposed to 25 nM of RocA for different durations (0, 1, 3, 6, 12, and 24 h), and then the expressions of cGAS, STING, pTBK1, TBK1, pIRF3, and IRF3 were detected by Western blotting analysis. B, A549, H1299, and H1975 cells were exposed to different concentrations (0, 12.5, 25, and 50 nM) of RocA for 24 h, and then the expressions of cGAS, pTBK1, TBK1, p65, and pp65 were detected by Western blotting analysis. C, A549, H1299, and H1975 cells were transfected with STING siRNA or negative control (NC) for 24 h and then exposed to 25 nM of RocA for 24 h, followed by the detection of STING, pTBK1, and TBK1 by Western blotting analysis. Data represented three independent experiments. E, A549, H1299, and H1975 cells were transfected with STING siRNA or NC for 24 h and then exposed to different concentrations (0, 12.5, and 25 nM) of RocA for 24 h, followed by the detection of CCL5 and CXCL10 by real-time PCR. F, A549, H1299, and H1975 cells were transfected with STING siRNA or NC for 24 h and then exposed to 25 nM of RocA for 24 h, followed by the analysis of NK cell migration. Data were pooled from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001.