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. 2022 Jan 1;18(2):585–598. doi: 10.7150/ijbs.65019

Figure 7.

Figure 7

RocA damages mtDNA and promotes the cytoplasmic release of mtDNA. A549, H1299, and H1975 cells were exposed to 25 nM of RocA or 5 µM of CPT for 24 h. A, The cells were stained with anti-γH2AX antibody and DAPI, and observed under laser confocal microscopy. B, The proportions of γH2AX+ cells in the cytoplasm. C, The proportions of γH2AX+ cells in the nucleus. D-F, A549, H1299, and H1975 cells were exposed to different concentrations (0, 12.5, and 25 nM) of RocA for 24 h, and then the expressions of CO1, ND1, and D-Loop in the cytoplasm were detected by real-time PCR. G, A549, H1299, and H1975 cells were exposed to different concentrations (0, 12.5, and 25 nM) of RocA or different concentrations (0, 6.25, and 12.5 µM) of CsA for 24 h, and then the expression of CO1 in the cytoplasm was detected by real-time PCR. Data represented three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, non-statistical significance.