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. 2022 Jan 1;18(2):717–730. doi: 10.7150/ijbs.64350

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Effect of apoptin on cell membrane and inhibition of HCT116 cellviability. (A) HCT116 cells were treated with Ad-mock, Ad-vp3 and Ad-vt at 100 MOI, and with crystal violet staining at 12, 24, 36, 48, 72 hours. (B) HCT116 cells were treated with Ad-mock, Ad-vp3 and Ad-vt at 100 MOI, the viability of cells was determined by the CCK8 assay. (C) The percentage of apoptosis (stained with Annexin V⁺/PI^-) or pyrotosis (stained with Annexin V⁺/PI⁺) was indicated at 36h. (D) A laser confocal microscope image (E) transmission electron microscopy. The red arrowheads indicate the emerging pore from the plasma membrane. (F) Hoechst staining of cells treated with Ad-vp3 and Ad-mock respectively showed the proportion of nuclear thickening and nuclear fragmentation.