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The interaction between apoptosis and pyroptosis induced by apoptin. (A) HCT116 cells were treated with Ad-vp3 and Ad-vt for 6h to 72h. The markers of pyroptosis and apoptosis were analyzed by western blot. (B) Western blot analysis of markers of pyroptosis and apoptosis with or without GSDME overexpression. (C) In the presence or absence of GSDME knockdown. (D) The quantification of positive apoptotic cells was assessed by the TUNEL assay, with or without GSDME knockdown.
