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. 2022 Jan 1;18(2):507–521. doi: 10.7150/ijbs.67770

Figure 2.

Figure 2

IGF2BP2 is a downstream target of HPV16 E6/E7 and is associated with tumor stage in patients with CC. (A) An enhanced volcano plot was generated using the OmicStudio tools at https://www.omicstudio.cn/tool. The red and blue dots represent upregulated and downregulated genes, respectively (fold change >2, p < 0.05, n=3). (B) A heat map was constructed based on the genes differentially expressed between the control and HPV16 E6/E7-knockdown cells. (C, D) qRT-PCR (C) and western blot (D) analysis of potential target genes and their products in SiHa and HeLa cells. (E) Differences in IGF2BP2 expression between CC tissue and normal tissue were analyzed by UALCAN (http://ualcan.path.uab.edu/), TNMplot (https://tnmplot.com/analysis/), GEPIA (cancer-pku.cn), and CESC microarrays (GSE63514). (F) The expression of IGF2BP2 in para-carcinoma tissues and CC tumor tissues at various clinical stages as determined by IHC analysis. Each value represents the mean ± SD for triplicate samples (Student t test). **P < 0.01, ***P < 0.001, and ****p < 0.0001.