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. 2022 Jan 1;18(2):507–521. doi: 10.7150/ijbs.67770

Figure 5.

Figure 5

The overexpression of IGF2BP2 partially rescued the HPV16/18 E6/E7 knockdown-mediated reduction in CC cell proliferation, metastasis, and aerobic glycolysis. (A) qRT-PCR-based evaluation of the mRNA levels of IGF2BP2 and those of downstream factors following the transfection of an IGF2BP2 expression plasmid. (B) The relationship between HPV16 E6/E7 and MYC was assessed using a RIP assay. (C) SiHa and HeLa cells expressing siHPV16/18 E6/E7 were transfected with IGF2BP2, after which the protein levels were determined using western blot. (D, E) Cell viability and clonogenicity were determined in siHPV16/18 E6/E7-expressing SiHa and HeLa cells transfected or not with oe-IGF2BP2 were assessed by CCK-8 assay (D) and colony formation assay, respectively (E). (F, G) Migration (F) and invasion assays (G) of siHPV16/18 E6/E7-expressing SiHa and HeLa cells transfected with oe-IGF2BP2 or oe-NC. (H) Cell proliferation was monitored using an EdU staining assay. (I-L) IGF2BP2 was overexpressed in SiHa and HeLa cells transfected with siHPV16/18 E6/E7, following which glucose uptake (I), lactate production (J), ATP levels (K), and intracellular ROS content (L) were determined. (M) The ECAR and OCR were detected in CC cells expressing NC, siHPV16/18 E6/E7+oe-NC, or siHPV16/18 E6/E7+oe-IGF2BP2. Each value represents the mean ± SD for triplicate samples (Student t test). **P < 0.01, ***P < 0.001, and ****p < 0.0001.