Figure 3.

Large-scale molecular phenotyping-compatible 3D imaging of cerebral vasculature. (A) Maximum projection of light-sheet fluorescence microscopic images of the entire vasculature in a SeeNet-treated brain (1.6× zoom, ×0.63 objective lens, numerical aperture: 0.15, working distance: 87 mm) into a single stacked photo. Casted vessels are shown in red. Arterioles immunolabeled with anti-α smooth muscle actin (anti-αSMA) are shown in green. Scale bar = 1 mm. (B) Maximum projection of confocal images of casted vasculatures from the pia to the hippocampus. A cortico-hippocampal vascular path is shown in magenta. Scale bar = 100 μm. (C) The anti-αSMA immunosignal in the same field is shown on a green scale. Scale bar = 100 μm. (D) A 3D-rendered view of the cortico-hippocampal vascular path. Reproduced with permission from Reference 104.