Table 1.

Advanced 3D imaging technology and characterizations in imaging of small mammals.

Advanced 3D imaging technology		Focus point
		Whole brain scale	Imaging depth	Living animals	Behaving animals
Light	Synchrotron radiation imaging	√		Absorption imaging
	Two-photon imaging		up to mm-level	√	√
	Light sheet microscopy		up to mm-level	some methods	some methods like SCAPE
	Imaging system combined serial sectioning	√
	Optical coherence tomography		up to mm-level	√	√
	Dynamic light scattering method		up to mm-level	√	√
Acoustics	Photoacoustic imaging		up to cm-level	√	√
Magnetics	MRI	√		√	√

Multimodal imaging: combined with the different techniques in the table above, or super-resolution microscopy, etc.

On the right side of the table are some separate focus points for imaging the brains of animal models, particularly rodents, including whether they are used for whole-brain imaging, the depth of imaging, and whether they are used in living or behaving animals. SCAPE: swept, confocally-aligned planar excitation. μCT: microcomputed tomography.