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. 2022 Jan 1;18(2):693–706. doi: 10.7150/ijbs.66760

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Preparation and characterization of Aβ42 species. (A) The protocol used for the preparation of LMW and HMW Aβ42 species from synthetic peptides. (B) Tris-tricine gel analysis of the size distribution of LMW and HMW Aβ42 species as probed by the 6E10 antibody. (C) Dot blot analysis of the presence of A11-positive species in both preparations. (D) MTT assay to measure the cytotoxicity of LMW and HMW Aβ42 species in mouse primary neurons. Data are expressed as the mean ± SD (* and ^#, p< 0.05 vs. 0 μM).