DAPK1 inhibition by C6 protects primary neurons against Aβ42 species-induced neuronal apoptosis and tau dysregulation. (A) and (B) Immunoblot analysis and statistics of cleaved caspase-3, cleaved PARP1 and DAPK1 function in WT primary neurons treated with 20 μM Aβ species alone or together with 2 μM C6 for 24 h. (C) and (D) Immunoblot analysis and statistics of total tau and tau phosphorylation at Thr231, Ser262 and Ser396 in WT primary neurons treated with Aβ species alone or together with C6 for 24 h. β-actin was used as an internal control. Data are expressed as the mean ± SD (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns, not significant).