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. 2022 Jan 7;12:138. doi: 10.1038/s41598-021-03947-9

Table 1.

The rate of H2O2 generation in subsarcolemmal mitochondria (SSM) from wild type and CPNS1 deletion with or without ISC–REP.

Mice Wild type CPNS1 deletion
Groups Time control (n = 7) ISC–REP (n = 8) Time control (n = 7) ISC–REP (n = 7)
Intact mitochondria Complex I substrates: Glutamate + Malate
H2O2 (pmol/min/mg) 84 ± 19 75 ± 22 80 ± 16 82 ± 24
Complex II substrates: Succinate
H2O2 (pmol/min/mg) 974 ± 419 280 ± 94* 437 ± 165 * 172 ± 62
Complex II substrates: Succinate + Rotenone
H2O2 (pmol/min/mg) 405 ± 63 394 ± 127 282 ± 98* 240 ± 77
Reverse flow-induced ROS generation
H2O2 (pmol/min/mg) 678 ± 357 148 ± 120*
Solubilized Mitochondria Complex I substrate: NADH
H2O2 (pmol/min/mg) 752 ± 63 1029 ± 214* 608 ± 160 630 ± 59

Mean ± SD. *p < 0.05 vs. corresponding WT time control. p < 0.05 vs. CPNS1 deletion time control. p < 0.05 vs. wild type ISC–REP. Reverse flow-induced ROS represents the difference between the rate of H2O2 generation using succinate alone as substrate and the rate of H2O2 generation using succinate as substrate in the presence of rotenone to block reverse electron flow.