Table 1.
Mice | Wild type | CPNS1 deletion | ||
---|---|---|---|---|
Groups | Time control (n = 7) | ISC–REP (n = 8) | Time control (n = 7) | ISC–REP (n = 7) |
Intact mitochondria | Complex I substrates: Glutamate + Malate | |||
H2O2 (pmol/min/mg) | 84 ± 19 | 75 ± 22 | 80 ± 16 | 82 ± 24 |
Complex II substrates: Succinate | ||||
H2O2 (pmol/min/mg) | 974 ± 419 | 280 ± 94* | 437 ± 165 * | 172 ± 62† |
Complex II substrates: Succinate + Rotenone | ||||
H2O2 (pmol/min/mg) | 405 ± 63 | 394 ± 127 | 282 ± 98* | 240 ± 77‡ |
Reverse flow-induced ROS generation | ||||
H2O2 (pmol/min/mg) | 678 ± 357 | – | 148 ± 120* | – |
Solubilized Mitochondria | Complex I substrate: NADH | |||
H2O2 (pmol/min/mg) | 752 ± 63 | 1029 ± 214* | 608 ± 160 | 630 ± 59‡ |
Mean ± SD. *p < 0.05 vs. corresponding WT time control. †p < 0.05 vs. CPNS1 deletion time control. ‡p < 0.05 vs. wild type ISC–REP. Reverse flow-induced ROS represents the difference between the rate of H2O2 generation using succinate alone as substrate and the rate of H2O2 generation using succinate as substrate in the presence of rotenone to block reverse electron flow.