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. 2022 Jan 7;12:49. doi: 10.1038/s41598-021-04417-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profiling of pluripotency regulating genes. (A–C) Heatmaps using log₂ TMM values of the normalized read counts of pluripotency regulating genes in CEFs and EGK.X blastoderms (A), CEFs and PGCs (B), and EGK.X blastoderms and PGCs (C) were prepared separately for better visualization. (D) Violin plots were prepared with the log₂ TMM values to visualize the overall expression of pluripotency regulating genes in CEFs, EGK.X blastoderms, and PGCs. (E–G) DEGs of pluripotency regulating pathway between the test samples. DEGs in EGK.X blastoderms compared with CEFs, DEGs in PGCs compared with CEFs, and DEGs in PGCs compared with EGK.X blastoderms were shown in (E–G), respectively. Green: significantly upregulated. Red: significantly downregulated. Yellow: unregulated. (H–J) GSEA of pluripotency regulating genes at CEFs versus EGK.X blastoderms (H), CEFs versus PGCs (I), and EGK.X blastoderms versus PGCs (J) conditions.