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. 2022 Jan 7;12:49. doi: 10.1038/s41598-021-04417-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TUNEL assay and γ-H2A.X immunocytochemistry in CEFs, EGK.X blastoderm cells (BCs), and PGCs treated with H₂O₂. The cultured CEFs, EGK.X blastoderm cells, and PGCs were treated with 1 mM H₂O₂ for 1 h (H₂O₂ 1 h) in one group. In three other groups, the cells were treated with 1 mM H₂O₂ for 1 h, and then incubated in fresh media without H₂O₂ for 3 h (repair 3 h), 6 h (repair 6 h) and 12 h (repair 12 h). After treatment, cells were separately subjected for TUNEL assay with TMR-red staining (left panel) and γ-H2A.X immunocytochemistry (right panel).