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. 2022 Jan 7;12:205. doi: 10.1038/s41598-021-04065-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Adhesion of gastric cancer cells on human peritoneal mesothelial cells (HPMC). HPMC were cultured with TGF-β as described in the legend of Fig. 2 and then fluorescein labelled NUGC-4 and MKN45 were added, incubated for 15 min on the mesothelial cell (MC) monolayer. After gentle washing with warmed media for 3 times, the number of NUGC-4 cells remaining attached were counted under a fluorescent microscope. In (A) and (B), MC monolayer was incubated with RGD peptide (40 μM), anti-β1 integrin mAb or isotype control mouse IgG (20 μg/ml) before the addition of tumor cells. In (C) and (D), MC monolayer was pretreated with miR-29b mimic or negative control as described in the legend of Fig. 2. Data show mean ± standard deviation in 1 of the 2 different experiments ***p < 0.001, ****p < 0.0001.