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. 2022 Jan 7;12:263. doi: 10.1038/s41598-021-04360-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PAW ultrasonic nebulization on SARS-CoV-2 infectivity. The figure depicts the results for a representative experiment. Serial dilutions of SARS-CoV-2 inoculum were adsorbed to Vero E6 cell monolayers for either 0.5 or 5 h before nebulization with different media. Nebulization was maintained for 10 min before washing with DMEM. Viral titers were then estimated by plaque assay. PBS nebulization was used as a negative effect control. Mean ± SD viral titers are shown. Two-way anova with Tukey’s post-hoc test. ****p<0.0001.; ns: non-significant. Generated with INKSCAPE 1.1 (www.inkscape.org).