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. 2022 Jan 7;12:263. doi: 10.1038/s41598-021-04360-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of plasma activated water on infectivity and inflammation caused by influenza PR8 H1N1 virus. (A) Results of inhibition of PR8 H1N1 virus replication in the canine kidney MDCK cell line obtained after infection with PR8 and subsequent treatment with bPAW. The figure depicted the PR8 nucleoprotein transcript level (arbitrary units, a. u.). (B) bPAW Inhibition of the internalization of the PR8 virus measured by nucleoprotein RNA transcripts (a. u.). A549 human lung cells were subjected to PR8 H1N1 infection for 1 h and subsequently treated with bPAW for 20, 60 and 90 min. (C) Study of inflammation produced by bPAW treatment in the A549 human lung cell line. The panel denoted the normalized level of interleukin 8 RNA transcripts. All figures represent normalized values (Mean ± SD). Statistical analysis was carried out using one-way anova with Tukey’s post-hoc test, *p<0.01; **p<0.001. Generated with INKSCAPE 1.1 (www.inkscape.org).