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. Author manuscript; available in PMC: 2022 Jan 8.
Published in final edited form as: Nature. 2021 Jun 3;595(7869):718–723. doi: 10.1038/s41586-021-03673-2

Fig. 1 ∣. Engineering of IgM and IgA1 neutralizing monoclonal antibodies.

Fig. 1 ∣

a, The IgG1 epitope residues are shown as spheres on the RBD. b, Illustration of antibody engineering from IgG1 into IgM and IgA1. c, d, SDS–PAGE (left) and native PAGE (right) (c) and size-exclusion chromatography (d) analysis showing monoclonal antibody assembly and purity. The gel images were from one experiment. HC, heavy chain; LC, light chain. e, ELISA binding to the RBD by the indicated isotypes of CoV2-14. Data are mean of duplicate wells. f, Antibody valency versus EC50 for the indicated isotypes. g, Neutralization of SARS-CoV-2 by the indicated monoclonal antibodies at 0.1 μg ml−1. Data are mean ± s.d. of triplicates. h, The EC50 and per cent neutralization of the three isotypes for CoV2-06 and CoV2-14 are plotted to illustrate different correlation patterns between binding and neutralization.