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. 2021 Dec 2;26(1):151–162. doi: 10.1111/jcmm.17065

FIGURE 3.

FIGURE 3

miR‐422a was the directly target of circ_0125310. A, Five miRNAs were screened in MCs transfected with LV‐circ_0125310. B, The expression level of miR‐422a was upregulated in MCs transfected with sh‐circ_0125310. C, The expression level of miR‐422a was decreased in kidney tissues with DN (T2D without DN=24, T2D with DN = 32). D, Expression levels of miR‐422a was downregulated in kidney tissues with macroalbuminuria group (microalbuminuria group = 12, macroalbuminuria group = 20). E, Expression levels of miR‐422a was downregulated in kidney tissues with high eGFR group (low eGFR group = 14, high eGFR group = 18). F, The expression level of miR‐422a was decreased in kidney tissues of the DN rat model. G, circ_0125310 in cell lysis was pulled down and enriched with circ_0125310 specific probe in MCs. H, miR‐422a was pulled down and enriched with circ_0125310 specific probe in MCs. I and J, Biotin‐coupled miR‐422a captured a fold change of circ_0125310 in the complex as compared with biotin‐coupled NC in biotin‐coupled miRNA capture. Then products were detected by agarose gel electrophoresis. K, The direct binding sites between circ_0125310 and miR‐422a were presented. L, Luciferase reporter assay was performed to confirm the direct binding relationship between circ_0125310 and miR‐422a