Fig. 6.
Full-length or N-terminal pejvakin expression does not induce pyroptosis. A Structural alignment of the molecular models of mouse GSDMA3NT (6CB8) (brown) with the model for the template generated human PJVKNT (cyan) (left panel). Structural alignment of human GSDMENT (brown) with human PJVKNT (Cyan) both generated from the mouse GSDMA3NT template-based model (middle panel). Structural alignment of mouse GSDMA3NT (6CB8) (brown) with the template-based model generated for jawless Chordate lancelet fish (Branchiostoma floridae) GSDMNT (Cyan) (right panel). B Measurement of LDH release, IL-1β release, and Yo-Pro-1+ uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express human full length PJVK, PJVKNT, full length GSDMD, or GSDMDNT. C Measurement of LDH release, IL-1β release, and Yo-Pro-1+ uptake in HEK293 cells that constitutively express the mature form of human IL-1β and transiently express lancelet GSDMNT (blue), shark PJVKNT, or GSDMENT (dark yellow) or mouse GSDMDNT (red). In B and C, each point represents an individual assay with at least n=3 to 6 different assays. *p<0.05, **p<0.01, and ***p<0.001; Kruskal-Wallis test with Dunn’s multiple comparison post-test