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Light microscopic study of PPD-stained axons 2 weeks following intravitreal administration of (A) PBS, (B) TNF, (C) 2 pmol netarsudil + TNF, (D) 20 pmol netarsudil + TNF, or (E) 200 pmol netarsudil + TNF. Scale bar: 10 µm. (F) Quantification of axon number; n = 5 to 8 per experimental group. *P < 0.05, **P < 0.0005.
