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Immunoblot analysis of (A) LC3-II, (B) p62, and (C) p-AMPK in optic nerve samples 1 week after intravitreal administration of 20 pmol netarsudil, PBS, TNF, or 20 pmol netarsudil + TNF; n = 6 to 8 per experimental group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
