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Immunohistochemical analysis of the optic nerve. (A) The p-AMPK immunoreactivity was colocalized with neurofilament positive fibers in PBS-treated optic nerve (arrows), and abundant p-AMPK immunoreactivity was found with neurofilament positive fibers 1 week after netarsudil treatment (arrows). Scale bar: 50 µm. (B) The p-AMPK signal intensity 1 week after injection of PBS or 20 pmol netarsudil; n = 3 per experimental group. *P < 0.05.
