Figure 3.

In the trigeminal nucleus of LGE rats, the α₂δ–1 subunit protein expression was increased on the LGE side at 2w and 8w. (A) The mRNA level of Cacna2d1, encoding the α₂δ–1 subunit, in the trigeminal nucleus at 8w. Each bar graph represents the mean ± SEM (n = 3). (B, C) Immunofluorescence images of trigeminal nuclei on the sham side (B) and the LGE side (C) at pre. α₂δ (green), NeuN (magenta), and DAPI (cyan) indicate α₂δ–1, neurons, and nuclei, respectively. Scale bars: 30 µm (upper panel); 10 µm (lower panel). (D, E) Immunofluorescence images of trigeminal nuclei on the sham side (D) and the LGE side (E) at 8w. (F) Summary of the expression levels of α₂δ–1 in the trigeminal nucleus of pre, 1w, 2w, and 8w. The vertical axis shows the RQ value of total fluorescence intensity (FI) on the LGE side normalized to that on the Sham side in the same section from the same animal. Each bar graph represents the mean ± SEM (n = 3). *P < 0.05, **P < 0.01 versus sham side (Dunnett's multiple comparison test).