Fig. 4. Effect of HMGB1, NC, BAF, and HMGA1 on integration activity.

Lane 1 shows the products of a reaction including only IN as a protein factor (A). Additional proteins were included in the integration mixture at the following concentrations: HMGB1, 40 nm, 80 nm, 320 nm, and 1.6 μm (A, lanes 2–5); NC, 50 nm, 100 nm, 250 nm, 500 nm, and 2 mm (A, lanes 6–10); BAF, 50 nm and 100 nm (B, lanes 11 and 12); and HMGA1, 70 nm, 140 nm, 350 nm, and 1.4 μm (B, lanes 13–16).