Table 1.
PCR primers
| Primer | Forward | Reverse |
|---|---|---|
| β-ACTIN | GGACCTGACCGACTACCTCA | GCGACGTAGCAGAGCTTCTC |
| CYP7A1 | GAAAGAGAGACCACATCTCGG | GAATGGTGTTGGCTTGCGAT |
| CYP27A1 | ACTGAAGACCGCCGATGAAAC | CAAAGGCGAATCAGGAAGGG |
| CYP4A21 | TTTTCCCGCTTGAGGAGTGC | ACTCGGTCTGTGTGTTGATGGA |
| CYP7B1 | AATACTTCCTCCCTTCTGCCC | GGGCAGAAGGGAGGAAGTATT |
| NTCP | ACTTTCGGAAACCTAAGGGACT | AAGAGCTTGCCCAGTGCAAAG |
| ILBP | CTCCTGCCTCATCCTTC | CATCGTAGTTCTTCTACTC |
| OST-α | TGTACAAGAACACTCGCTGC | GAACACACACACTATCGTGGG |
| ASBT | ATAATGGGATGCTGTCCAGG | TAGATTAAGAGGCACAGCGG |
| BSEP | TTTCATTCAGCGCCTGACCA | ACTCCAATGAGAGGGCTGAC |
| FGF19 | CTGGGCCGCACGTGCACTAC | GGGCCCGTCTGAGTGGATGCG |
| SHP | GCCTACCTGAAAGGGACCAT | CAACGGGTGTCAAGCCTTTA |
| FXR | TTTGTGTCGTTTGCGGAGAC | GTTGCCCCCATTTTTACACTTG |
| KI67 | AGTCTGTAAGGAAAGCCACCC | ACAAAAGCCAAGCAGACAGG |
| SOX9 | CGGTTCGAGCAAGAATAAGC | GTAATCCGGGTGGTCCTTCT |
| FGFR1 | GGTTGTAGCAGTATTGCAG | CGGACCTCTATATGTCATTG |
| FGFR4 | CTACAAGAAAAGAAGCAACG | TGTAGACTCTGTCAAACAAG |
| KLB | AAGGCCTTCCAAGACTACGC | GCGCTCGTAGATGTCACTCA |
| GATA-4 | CGGGAAGAAACTTGAAGTCG | TCCCATAAAATCCAGCACCT |
ASBT, apical sodium-dependent bile acid transporter; BSEP, bile salt export pump; CYP7A1, cytochrome P450 section 7 family A member 1; CYP27A1, cytochrome P450 family 27 subfamily A member 1; CYP4A21, cytochrome P450 family 4 subfamily A member 21; CYP7B1, cytochrome P450, family 7, subfamily B, member 1; FGF19, fibroblast growth factor 19; FGFR1, fibroblast growth factor receptor 1; FGFR4, fibroblast growth factor receptor 4; FXR, farnesoid X receptor; GATA-4, GATA binding protein 4; ILBP, ileal lipid binding protein; KI67, antigen KI-67; NTCP, Na-taurocholate cotransporting polypeptide; OST-α, organic solute transporter α; SHP, small heterodimer partner; SOX9, SRY-box transcription factor 9; KLB, β-klotho.