Table 1.

The reaction master mixture for cDNA preparation.

Reagents	Volumes for 50 μl
Denaturized RNA	15 μl
Random hexameter primers 3μg/μl	2.0 μl
10 mM dNTP Mix	5 μl
5x cDNA synthesis buffer	10 μl
RNase OUT (40 U/μl)	2.5 μl
Thermo Script RT (15 units/μl)	2.5 μl
DEPC-treated water	14.8 μl
Total	50 μl