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. 2022 Jan 8;10:4. doi: 10.1186/s40478-021-01285-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Functional conservation of human BIN1 isoforms in Drosophila. a Scheme of cerebral human BIN1 isoform1 (BIN1iso1), muscular human BIN1 isoform8 (BIN1iso8), ubiquituous human BIN1 isoform9 (BIN1iso9) and Drosophila BIN1, called Amphiphysin, isoformA (dAmphA) for which transgenic lines were generated. b Western blot analysis of Amph and BIN1 expression in dAmph^{MI08903-TG4.0/+}, dAmph^{MI08903-TG4.0/5E3} and dAmph^{MI08903-TG4.0/+}; UAS-BIN1iso8 fly thorax. dAmphA, dAmphB and dAmphC were expressed in the heterozygous dAmph^MI08903/+ flies, whereas they were not detected in dAmph^MI08903/5E3 flies (*background staining). BIN1iso8 was expressed in dAmph^{MI08903-TG4.0/+}; UAS-BIN1iso8 flies. c Analysis of the climbing locomotor activity of 2 day-old flies with the indicated genotype. dAmph^{MI08903-TG4.0/5E3} flies exhibited a similar low climbing score as the null dAmph^5E3/5E3flies. Expression of BIN1iso8 rescued the locomotor defects of dAmph^{MI08903-TG4.0/5E3} flies (from left to right n = 10, 5, 49, 67, 26, ANOVA F-value = 66.15, Df = 4, p = 2.37 × 10⁻³⁸ with post-hoc Tukey, n.s. not significant). d Visualization of outer photoreceptor neuron rhabdomeres by cornea neutralization in 2-day-old flies expressing mCherry (as a control), BIN1iso1, BIN1iso8, BIN1iso9 and dAmphA under a GMR driver (attP2 is a control with an empty attP2 landing site and no UAS construct). While each ommatidium contained 6 outer photorceptors organized in a trapezoid shape (yellow circles) in the two control conditions, BIN1 isoforms and dAmphA expression resulted in a strong alteration in the number, shape and trapezoid organization of the rhabdomeres with a stronger effect for BIN1iso1 and BIN1iso9 high-expressing lines. e Immunofluorescence of whole-mount pupal retina expressing Luciferase (as a control), BIN1iso1, BIN1iso8, BIN1iso9 or dAmphA. They were labelled for the plasma membrane neuronal Na/K ATPase and F-actin. Contrary to the control, BIN1iso1, BIN1iso8, BIN1iso9 and dAmphA induced a strong accumulation of F-actin at the level of the rhabdomere