Fig. 1.

EGFR-AS1 is highly expressed in CC cells and EGFR-AS1 knockdown hampers CC cell growth. a EGFR-AS1 expression in CC cell lines and human normal cervical cell line was detected by qRT-PCR. b The transfection of sh-EGFR-AS1#1/2 was detected via qRT-PCR. c, d Colony formation and EdU assays were performed to evaluate the effect of EGFR-AS1 knockdown on CC cell proliferation. e, f The apoptosis in EGFR-AS1 silenced cells was tested via flow cytometry analysis and TUNEL assay. g, h Western blot assay and transwell assay were carried out for assessing cell migration and invasion. *P < 0.05; **P < 0.01. Scale bar 100 µm