FIGURE 3.

Effects of MMDaV RepA deletion mutations on RepA cell death‐inducing activity. (a) Schematic representation of wild‐type (WT) RepA and the eight RepA deletion mutants. The predicted conserved domains were shown as indicated. The catalytic domain contains the conserved motifs I (DNA binding), II (metal binding), and III (DNA cleavage and ligation). A and B in the C‐terminus of RepA represent the Walker A and Walker B motif, respectively. (b) Analysis of the cell death‐inducing activity of RepA deletion mutants. Top panels, visualization of the cell death response in agroinfiltrated Nicotiana benthamiana leaves under bright light at 3 days postinfiltration (dpi). Bottom panels, the representative images of agroinfiltrated N. benthamiana leaves stained with 3,3′‐diaminobenzidine (DAB) at 3 dpi