TABLE 2.
The primers used in the experiment
| Primer | Sequence 5′–3′ | Purpose |
|---|---|---|
| CsSP1‐F1 | GAGGTCGTGGGACTATCTTATAGCCAG | Amplification of upstream fragment of CsSP1 |
| CsSP1‐R1 | TTGACCTCCACTAGCTCCAGCCAAGCCTTGAAGTCCGGTACGCTGTGAATGAA | |
| CsSP1‐F2 | ATAGAGTAGATGCCGACCGCGGGTTCCTAGCAATACCAGATGTAAC | Amplification of downstream fragments of CsSP1 |
| CsSP1‐R2 | GATCCCATGGCCTGCTCTAAGA | |
| CsSP1‐NF | GTGGCTTCCGCACCTGCTACA | Amplification of internal fragment of CsSP1 |
| CsSP1‐NR | CTTCTCCGACCCTGGATATTG | |
| CsSP1‐PF | TTAGCCGCAGATGACCTTG | Generation of knockout mutant |
| CsSP1‐PR | CCGCAAGTGCATGGGTGTAAG | |
| HYG‐F | GGCTTGGCTGGAGCTAGTGGAGGTCAA | Amplification of hygromycin resistance gene |
| HY‐R | GTATTGACCGATTCCTTGCGGTCCGAA | |
| YG‐F | GATGTAGGAGGGCGTGGATATGTCCT | |
| HYG‐R | GAACCCGCGGTCGGCATCTACTCTAT | |
| H855R | GCTGATCTGACCAGTTGC | |
| H856F | GTCGATGCGACGCAATCGT | |
| PVX‐CsSP1‐F | CACCAGCTAGCATCGATTCCCGGG ATGCGTTTCATCATCTCC | Construction of PVX carrier |
| PVX‐CsSP1‐NS‐F | CACCAGCTAGCATCGATTCCCGGGATGGAGGTGGCTTCCGCA | |
| CsSP1‐GFP‐R | CTCGCCCTTGCTCACCATGGTCCCGCGGTTTGGC | |
| GFP‐CsSP1‐F | GCCAAACCGCGGGACCATGGTGAGCAAGGGCGAG | |
| PVX‐GFP‐F | CACCAGCTAGCATCGATTCCCGGGATGGTGAGCAAGGGCGAG | |
| PVX‐GFP‐R | CGCAATCTCTAGAGGATCCTTGTACAGCTCGTCCAT | |
| CsSP1‐PYIP‐F | GCTTGATATCGAATTCCTGCAGGATCATTTCCATCATCGCGCT | Construction of PYIP carrier |
| CsSP1‐PYIP‐R | GAAAATAAAGATTCTCGGTCCCGCGGTTTGGCTGTTC | |
| PB‐CsSP1‐F | CATTTACGAACGATAGGGTACCATGCGTTTCATCATCTCCATATTC | Construction of PB carrier |
| PB‐CsSP1‐R | GGTAAGGCCTACTAGTGGATCCGGTCCCGCGGTTTGGCTGTTC | |
| pSUC‐CsSP1‐F | AAGCTCGGAATTTTAATTAAATGCGTTTCATCATCTCCATA | Construction of pSUC carrier |
| pSUC‐CsSP1‐R | CGACTCACTATAGGGAGAACGGTCCCGCGGTTTGGCTGTTC | |
| CsSP1‐QRT2‐F | CAGCTCTTGCATCTGCTA | Expression of CsSP1 |
| CsSP1‐QRT2‐R | TCTTCTCCGACCTTGAG | |
| RT‐Actin‐F | GTATGGGCCAAAAGGACTCA | Internal reference primers of B. sorokiniana |
| RT‐Actin‐R | CACGCAGCTCGTTGTAGAAG | |
| TaPR1‐F | CTGGAGCACGAAGCTGCAG | Expression of PR gene in wheat |
| TaPR1‐R | CGAGTGCTGGAGCTTGCAGT | |
| TaPR2‐F | CTCGACATCGGTAACGACCAG | |
| TaPR2‐R | GCGGCGATGTACTTGATGTTC | |
| Actin‐PR‐F | GGAAAAGTGCAGAGAGACACG | Internal reference primers from TaBs109G1 clone of wheat |
| Actin‐PR‐R | TACAGTGTCTGGATCGGTGGT | |
| TaPAL‐F | TTCGATTTGCCACCAAGTC | Expression of PAL gene in wheat |
| TaPAL‐R | GTGCCTTGGAAGTTGCCAC | |
| CsBrlA‐F | TCCCGAAGCTATGGTGGATC | Expression of conidiation‐related genes |
| CsBrlA‐R | CTGGCTGGAGGTTGATGGTA | |
| CsMedA‐F | TGGTGGGAAGAAAAGGACGA | |
| CsMedA‐R | TCGCTGTCTTGCTTTGCTTT | |
| CsStuA‐F | ACCCAAAGACAGAGATGGCA | |
| CsStuA‐R | ACTCATTGCCGTGATCATGC | |
| CsSP1‐NLS‐F | GCCAAACCGCGGGACCCAGCCTAAGAAGAAGAGAAAGGTTGGAGGAATGGTGAGCAAGGGCG | Construction of CsSP1 location‐based signal mutants |
| CsSP1‐NES‐F | GCCAAACCGCGGGACCAACGAGCTTGCTCTTAAGTTGGCTGGACTTGATATTAACAAGATGGTGAGCAAGGGCG | |
| CsSP1‐mNES‐F | GCCAAACCGCGGGACCATGCTTCAAGCTCCTCCTGCTGAAAGAGCTACTCTTATGGTGAGCAAGGGCG | |
| PB‐GFP‐R | GGTAAGGCCTACTAGTGGATCCCTTGTACAGC TCGTCCATGC | |
| TaEF‐1α‐F | CAAGGGTGTGGAGAAGAAGG | Expression of a constitutive gene of wheat |
| TaEF‐1α‐R | AGCAGACATAGATGGATTCAGG |