Parents’ age, occupation, education, marital status, economy (mothers, fathers) Parents’ place of residence, housing Parents’ history of cardiovascular diseases in a family Parents’ history of other diseases Wantedness of the pregnancy Birthweight of previous children Maternal: weight, height, miscarriages, weight gain during pregnancy, age at menarche, stillbirths, parity, blood pressure (highest reading in 2-4th and 5-9th month), smoking 1 year before pregnancy and during pregnancy (no. of cigarettes), working during pregnancy, workload, Hb in the 3rd, 7th and 9th gestational month and before birth, oedema, albuminuria, certain infections, depression and subjective well-being during pregnancy, medication with type and dosage
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Birthweight Birth length Gestational age Placental weight and abnormalities, umbilical cord Mode of delivery Apgar score Congenital abnormalities Detailed information on neonatal hospital admission and diagnoses
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Development (e.g. standing, walking, talking, neurological abnormalities) Weight (also at 6 months) Height (also at 6 months) Head circumference Vitamin supplementation Diseases, hospitalizations, outpatient visits (causes) Medication Vaccination
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Weight, height, waist-hip circumference, leg length Physical activity Smoking, alcohol drinking, drugs Schooling Family structure Social mobility Parental occupation Long-term diseases (asthma, atopy, diabetes) Central nervous infections Brain damage Parental history of chronic diseases
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| 31 years |
46 years |
31 years |
46 years |
| Postal questionnaire n = 8690 |
Postal questionnaire n = 7146 |
Clinical examination n = 6007 |
Clinical examination n = 5832 |
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Marital status, family structure Migration Education, occupation and working life, income Smoking, alcohol drinking, medication, nutrition Cardiovascular disease or high blood pressure (diagnosed by a physician) Long-term diseases, ISAAC questions about asthma, atopy and wheezing Age at menarche Birthweight of own children Information on women’s own pre-eclampsia and deliveries Family history of chronic diseases
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Marital status, family structure Migration Education, occupation, working life, income, risk-taking Smoking, alcohol drinking, medication, nutrition Cardiovascular disease or high blood pressure (diagnosed by a physician) Long-term diseases, ISAAC questions about asthma, atopy and wheezing Life satisfaction, reserves of strength, sleep Pregnancy, endometriosis, pre-eclampsia, PCOS, infertility Cognitive tests (Paired Associates Learning, PAL)
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Weight, height, waist-hip circumference Blood pressure, pulse (a mercury sphygmomanometer) Physical fitness (step test) Back muscle strength Compression force of hands (hand grip dynamometer) Skin allergy tests (prick) Spirometry (Vitalograph P-model spirometer) Bone density (DTX 200, Osteometer Meditech) (n = 1000)
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Weight, height, waist-hip circumference, bioimpedance (InBody 720) Blood pressure (brachial), pulse (Omron M10) Physical fitness with 2-week accelerometry measurements (Polar RX800RC, Hookie AM20) Skin allergy tests (prick) Spirometry (MasterScreen Pneumo, Spira) 15-lead ECG (CAM14 GE Healthcare) Heart rate variability (Polar RX800RC. Polar WearLink belt), step-test, brachial blood pressure (in sitting and standing positions), baroreflex measurement and breathing frequency Back muscle strength Pressure pain threshold (algometer, Somedic), thermal perception thresholds and tolerance (modular sensory analyser, Somedic) Spinal and ankle movement tests, low back movement control tests (SpinalMouse Med Pro) Compression force of hands (hand grip dynamometer) Glucose tolerance test Eye examination (vision, refraction, intraocular pressure, thickness of cornea, field of vision, eye photography), randomized for half of the cohortb
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31 years biological samples and analyses
Maximum n = 6007
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46 years biological samples and analyses
Maximum n = 5832
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46 years extended study protocol for Oulu area |
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Biological samples collected: plasma, serum, EDTA-blood (DNA extracted) Laboratory measurements such as blood count, high-sensitivity CRP, glucose, insulin, triglycerides, total LDL-, HDL-cholesterol, vitamins C and D GWAS (n = 5402) NMR metabolomics (n = 5739) Methylation (n = 807) Telomers (n = 1450)
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Biological samples collected: plasma, serum, EDTA-blood (DNA extracted), PAXGene for RNA, cells, hair, saliva, faeces, urine Laboratory measurements such as blood count, high-sensitivity CRP, glucose, insulin, triglycerides, total LDL-, HDL-cholesterol, vitamin D, S-T4V, S-TSH, S-TPOAb, cortisol NMR metabolomics (n = 5800, OGGT n = 4900) Methylation (n = 760) Telomers (n = 5783) Immunoglobulins (n = 5774)
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Echo and carotid ultrasound (Vivid E9, GE Healthcare) (n = 1156) Dermal examination by physician (n = 1930) X-ray of knee, ankle, foot, (Simax/Fuji FCR XG-1) and OPTG (Orthopantomoraph OP100) (n = 1975) Lumbar MRI (1.5 T imaging Signa HDxt, General Electric) (n = 1534) Oral health: dental caries, periodontitis, occlusion, temporomandibular disorders, facial structures (3 D-imaging of mouth and teeth, Cadent iTero3D) (n = 1964)
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