Figure 1.

Biological characteristics test of the S06004ΔspiCΔrfaH. (A) Growth curves of the S06004ΔspiCΔrfaH mutant. The S06004ΔspiCΔrfaH and S06004 were grown in the LB broth at 37°C with shaking at 180 rpm for 16 h, and the OD₆₀₀ values were determined every hour. (B) Rough phenotype characteristics of the S06004ΔspiCΔrfaH mutant. Agglutination assay was performed using the O₉ MAb and acriflavine. Images were taken within 5 min.