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The expression of cytokines in the spleen after the immunization. The mRNA expression of IL-2, IL-4, and IFN-γ was measured by the qRT-PCR at 3, 7, and 14 DPI. And the fold change referred to the data compared with the blank control group. Data are presented as mean ± SEM. ∗P < 0.05, ∗∗P < 0.01.
