Figure 2.

Generation of inducible vascular EC-specific Twist1 overexpression mice

A: Targeting strategy for generating Tg-Twist1^iEC+ mice. B: Genotyping of mouse models using PCR methods. C: Analyses of Twist1 mRNA expression in lungs from WT and Tg-Twist1^iEC+ mice by RT-PCR. GAPDH was used as a loading control. D: Analyses of Twist1 mRNA expression in lungs from WT and Tg-Twist1^iEC+ mice by RT-qPCR, showing that Twist1 was overexpressed in Tg-Twist1^iEC+ mice. n=6; two-tailed t-test; ^***: P<0.001.