TABLE 2.
Primer | Sequence (5′→3′) | Gene | Nucleotide positionsa |
---|---|---|---|
F16 | TTAAAACAGCCTGTGGGTTG | 5′NTR | 1–20 |
F8 | AAACACGGACACCCAAAGTA | 5′NTR | 548–564 |
252 | GGCCCCTAGATGCGGCTAA | 5′NTR | 452–470 |
253 | GATACYTGAGCNCCCAT | 5′NTR-VP4 | 742–758 |
68 | GGGACCTTCCACCACCANCC | VP2 | 1177–1196 |
008 | GCRTGCAATGAYTTCTCWGT | VP3 | 2383–2402 |
011 | GCICCIGAYTGITGICCRAA | 2A | 3301–3320 |
012 | ATGTAYGTICCICCIGGIGG | VP1 | 2874–2893 |
Based on CVB3 (GenBank accession number M33854).