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. 2022 Jan 9;6(1):e374. doi: 10.1002/pld3.374

FIGURE 2.

FIGURE 2

Analysis of osa‐MIR171f‐overexpressing transgenic plants and knockout mutants in response to drought stress. (a) Expression levels of osa‐mature‐microRNA171a‐f (osa‐miR171) in osa‐MIR171f‐overexpressing (OE) transgenic plants and knockout (K/O) mutants. The expression level was assessed by Northern blot analysis. U6 transcript levels show equal loading of RNA. Total RNA was prepared from the 4‐week‐old plants. Osa‐miR171/U6 value was calculated by band image analysis. (b) The phenotype of osa‐MIR171f‐OE plants and K/O mutants during drought stress. Four‐week‐old plants from four independent lines of osa‐MIR171f‐OE plants (2, 3, 5, 8), the K/O mutant (2), and non‐transgenic (NT) controls were exposed to drought stress for 2 days, followed by re‐watering for 7 days. (c) Measurement of soil moisture contents (mV). Data represent mean value ± SD of 10 measurements performed in different regions of the soil. (d), Thermal imaging of osa‐MIR171f‐OE plants (2, 3, 5, 8), K/O mutants (2), and NT plants. Different leaf temperatures are expressed as different colors: lower in blue and higher in red. (e) The survival rate of osa‐MIR171f‐OE plants (2, 3, 5, 8), K/O mutants (2), and NT plants 7 days after re‐watering. Data represent mean value + SD (n = 30)