Figure 1.

Rapid amplification of the short-length nucleic acid targets using independent temperature modules with large temperature differences and optimized programs. (A) The PCR tube was moved manually between the independent high (120 °C) and low (40 °C) temperature modules as the reaction mixture reached the setting temperature. (B) Program 1 and 2 were performed on the normal PCR instrument; Program 3 was performed on the independent temperature modules. C: 1, 2, 3 represented the product of Program 1, 2 and 3. M: 100 bp marker.