Figure 3.

SARS-CoV-2 amplification reaction condition optimization in the novel ultra-fast real-time PCR instrument. The rapid test conditions including the reaction time of reverse transcription (A), pre-denaturation (B) and cycling conditions (C,D) in the ultra-fast real-time PCR instrument were optimized. H, M, and L represented high, medium and low concentration of SARS-CoV-2 RNA. Standard represented the standard test of SARS-CoV-2. Each experiment was repeated independently three times, and the mean values and standard deviations are shown. SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.