Fig. 1.

Phylogenetic tree comparing the CampyICE Cas9 proteins with other Campylobacter and Helicobacter Cas9 proteins. The CampyICE1 Cas9 protein (blue) is distinct from the previously described Cas9 proteins of C. jejuni and C. coli (red), other Campylobacter species (green), and selected Helicobacter species (black). C. jejuni subsp. doylei is shown as C. doylei. The tree was drawn using the neighbour-joining method based on an alignment with the mega7 muscle plugin. Bootstrap values are indicated at branches which scored >95 %, based on 500 iterations using mega7, using the JTT matrix and pairwise deletion. Bar, the number of amino acid substitutions per site. An alignment of a subset of Cas9 proteins with domain annotation is provided in Fig. S1.