Fig. 6.

Shiga-toxin expression, production and toxicity of strain 9000 structural variants. The chromosomal location of all differentially expressed genes in Z1723 (upregulated, red; downregulated, blue) are mapped to the reference strain 9000 genome (a). Prophage (purple blocks), the 220 kb region of duplication (green), ΦStx2a and ΦStx2c regions are highlighted. Expression of stx2a (b) total Stx toxin production (c) and Vero cell toxicity Stx (d) was measured for trial 2 strains Z1723, Z1766 and Z1767 in M9 media. Mean values+/- sem of four biological replicates (n=4) are shown for each assay. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.