FIGURE 4.

Identification and verification of proteins differentially present in the testes, as induced by chronic stress. A, The 13 most differentially expressed spots were identified as 10 proteins by MALDI‐TOF‐MS. A functional protein association network constructed by STRING showed a strong protein interaction network centred on Atp5a1. B, Western blot analysis of Atp5a1, Eno1, Pkm2, Ywhaz and Uqcrc2 in testis tissue on day 21 showed results consistent with the proteomics findings (n = 3 per group). C, The positive expression area of Atp5a1 was mainly distributed in the Leydig cells of testis tissue on day 21 (red arrow, bar = 50 µm), and the H‐score demonstrated a significant difference in the stressed group versus the control group (p < 0.05, n = 6 per group)