FIGURE 5.

LOXL1‐AS1 knockdown represses malignant cell behaviours and vasculogenic mimicry (VM) in glioma by upregulating miR‐374b‐5p. A–H. The data are displayed as mean ± standard deviation (n = 3, each group). A. miR‐374b‐5p expression is regulated by LOXL1‐AS1. negative controls (NC); **p < 0.01 vs. LOXL1‐AS1(+)NC; ##p < 0.01 vs. LOXL1‐AS1(−)NC. B. LOXL1‐AS1 expression is regulated by miR‐374b‐5p. **p < 0.01 vs. pre‐NC; ##p < 0.01 vs. anti‐NC. C. The sequence mediating binding between LOXL1‐AS1 and miR‐374b‐5p as well as the mutant sequence is shown. D. The relative luciferase activities after the co‐transfection of pre‐miR‐374b‐5p and LOXL1‐AS1‐Mut or LOXL1‐AS1‐Wt are shown. **p < 0.01 vs. LOXL1‐AS1‐Wt+pre‐NC. E. The expression level of matrix metalloproteinase 14 (MMP14) is regulated by co‐transfection of LOXL1‐AS1 and miR‐374b‐5p. **p < 0.01 vs. LOXL1‐AS1(−)NC+pre‐NC. F–H. Cellular proliferation, migration, invasion and VM results confirm the co‐operative action of LOXL1‐AS1 and miR‐374b‐5p transfection in glioma. **p < 0.01 vs. LOXL1‐AS1(−)NC+pre‐NC. Scale bars indicate 100 μm in the migration and invasion assays and 200 μm in the tube formation assay