FIGURE 5.

Silencing of IGF2BP3 impairs AKT/mTOR signalling. (A, B) Western blot analysis was performed to evaluate the effect of IGF2BP3 knockdown on the protein expression of p‐AKT, AKT, p‐mTOR and mTOR. (C, D) Immunohistochemistry (IHC) staining was used to detect AKT, p‐AKT, E‐cadherin and vimentin expression in xenografts. All cell images were magnified 400 times. Differences between groups were assessed using two‐tailed t tests or one‐way analysis of variance (ANOVA). (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)