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. 2021 Dec 7;26(2):375–384. doi: 10.1111/jcmm.17089

FIGURE 5.

FIGURE 5

Inhibition of EZH2 recovers the osteogenic differentiation capacity of old BMSCs. (A) Real‐time RT‐PCR of Foxo1, Cat and Sod2 expression in old BMSCs treated with DMSO (as the vehicle control) or DZNep, a small molecule blocking the function of EZH2. (B) Fluorescence assay and FCM analysis of ROS in old BMSCs treated with DZNep or DMSO. (C) (I) ALP staining of old BMSCs treated with DZNep or DMSO after 7 days of osteogenic induction. n =3. (t test) (D) Alizarin red staining of old BMSCs treated with DZNep or DMSO after 14 days of osteogenic induction. n = 3. (t test) (E) Real‐time RT‐PCR analysis of Runx2 and Ocn mRNA levels in old BMSCs treated with DZNep or DMSO after 14 days of osteogenic induction. n = 3. (t test) (F) Histological analysis of transplants of young BMSCs or old BMSCs treated with DMSO or DZNep after 1month of subcutaneous transplantation. n = 4. (ANOVA) Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. NS, no significance. n, the number of biological replicated