FIGURE 1.

Arsenic trioxide (ATO) can induce apoptosis in SH‐SY5Y cells, and necroptosis, autophagy, NF‐ƘB and MAPKs signalling pathways are not involved in ATO‐induced apoptosis. (A) SH‐SY5Y cells were treated with indicated concentration of ATO for different hours. Cell survival was determined by measuring ATP levels. Results are representative of three independent experiments. (B) SH‐SY5Y cells were treated for indicated hours with 10 µM ATO. The cells were lysed and immunoblotted with caspase‐3 and GAPDH antibodies. (C–E) SH‐SY5Y cells were treated with indicated reagents. Cell survival was determined by measuring ATP levels. Results are representative of three independent experiments. *p < 0.05, **p < 0.01, compared between the cells treated with 10 µM ATO and Ctrl group