TABLE 1.
Primers used in PCR amplification and sequencing of glpQ
Primer | Primer sequence (5′-3′) |
---|---|
glpQ F+1 | GGGGTTCTGTTACTGCTAGTGCCATTAC |
glpQ F−1 | CAATTTTAGATATGTCTTTACCTTGTTGTTTATGCC |
glpQ R−1 | GCACAGGTAGGAATGTTGGAATTTATCCTG |
glpQ R−2 | CAATACTAAGACCAGTTGCTCCTCCGCC |
Br-R2 | GTTGCTCCTCCGCCAATTATTATTAAGTC |
Br-glpQ RBS | GAGAGGATAAATTAATGAAATTCAAATTAACAATG |
M13 reverse | GGAAACAGCTATGACCATG |
T7 | GTAATACGACTCACTATAGGGC |
Br GlpQ fus 5′ | GCCGCTCGAGGAAAAGAAAATGCAAAAATAAATAAAAAATC |
Br GlpQ fus 3′ | GGCGGATCCGCTTGACCAGTTGCTCCTCCGC |