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. 2021 Dec;9(23):1719. doi: 10.21037/atm-21-2947

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Phosphorylated Nrf2 reduced apoptosis. (A-C) Western blot analysis showing the protein expression of Nrf2 and P-Nrf2 in TBHQ-treated cells following hypoxia and reoxygenation. **, P=0.0041; *, P=0.0256; n=3. (D-F) Flow cytometry showing the effects of TBHQ on the apoptosis of H9C2 cells after hypoxia and reoxygenation. **, P=0.0085; n=3. (G-K) Flow cytometry displaying the influence of TBHQ on the cell cycle of H9C2 cells after hypoxia and reoxygenation. ***, P<0.0001; **, P=0.0011; n=6. MH/R, myocardial hypoxia reperfusion; Nrf2, nuclear factor erythroid 2-related factor 2; P-Nrf2, phosphorylated Nrf2; TBHQ, tertiary butylhydroquinone.